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Inferring the origin, population structure, and demographic history of a species is a major objective of population genetics. Although many organisms have been analyzed, the genetic structures of subdivided populations are not well understood. Here we analyze Drosophila ananassae, a highly substructured, cosmopolitan, and human-commensal species distributed in the tropical, subtropical, and mildly temperate regions of the world. We adopt a multilocus approach (with 10 neutral loci) using 16 population samples covering almost the entire species range (Asia, Australia, and America). Analyzed with our recently developed Bayesian method, 5 populations in Southeast Asia are found to be central, while the other 11 are peripheral. These 5 central populations were sampled from localities that belonged to a single landmass (“Sundaland”) during the late Pleistocene (∼18,000 years ago), when sea level was ∼120 m below the present level. The inferred migration routes of D. Ananassae out of Sundaland seem to parallel those of humans in this region. Strong evidence for a population size expansion is seen particularly in the ancestral populations. PATTERNS of genetic variation within and between populations contain information about the origin, population structure, and demography of a species. To understand population differentiation and the forces influencing it, a useful approach is to analyze DNA sequence variation at multiple loci. This is motivated by the notion that population structure and demography affect the entire genome in a similar way, whereas other evolutionary forces (including selection) leave footprints at individual genes (; ). This approach has been used for several species, including humans (; ), Drosophila (; ), and the house mouse (). To varying degrees, genetic differentiation exists in every species. It influences the estimation of population genetic parameters and complicates the analysis of molecular variation. However, with simplified assumptions, considerable progress in modeling population subdivision has been made in recent years ( e.g., ). Using these methods, it is now possible to distinguish central (ancestral) from peripheral (derived) populations without applying phylogenetic methods (). Drosophila ananassae is one of the very few Drosophila species with a cosmopolitan distribution and is present in most of the tropical, subtropical, and mildly temperate regions of the world (). It lives in close association with humans throughout the entire species range (), being most abundant in South and Southeast (SE) Asia, including some southern islands of Japan, Polynesia, Australasian regions, and America (;; ). This species has likely originated in SE Asia and subsequently dispersed to other parts of the world, possibly through human activity (). Previous population genetic studies using DNA sequence polymorphism provided evidence for significant genetic structure in this species (). On the basis of these analyses, D. Ananassae appears to be an excellent organism for analyzing population subdivision. Here we adopt a multilocus approach using 16 populations covering almost the entire species range to infer the population structure of D. Analyzing putatively neutral nuclear DNA sequence polymorphisms from 10 independent loci, we are able to distinguish between central and peripheral populations. We confirm the SE Asian origin of D. Ananassae and delimit its ancestral species range to a region that was a well-connected single landmass (Sundaland) until the late Pleistocene [∼18,000 years ago (18 KYA)], when sea level was lower than today by ∼120 m. In addition, we study the demographic history of D. While the central subpopulations deviate most clearly from the neutral, constant-size equilibrium model, the population as a whole appears to be expanding. Population samples and marker fragment identification: We have sampled 160 isofemale lines from 16 locations in Asia, Australia, and America covering almost the entire species range of D. Matlab 2010 full crack 64 bit. Admix Model Ds-425 Manual Software Free DownloadAnanassae (). The abbreviated names of the sampling locations (as given in ) are used throughout this article. Since information on genomic DNA is limited in D. Ananassae and D. Melanogaster is a closely related species with a completely known genome sequence, we used genomic information of the latter species to identify our marker fragments. We chose introns from random genes located in the normal- to high-recombination regions of the X chromosome of D. Melanogaster and tested for amplification in D. PCR primers were designed in exons flanking these introns. For each sequenced fragment, the DNA sequences of the flanking exons were aligned with those of the corresponding fragment of D. 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Nine fragments (termed L2– L10; see online supplementary at ) of these specifications and a part of intron 1 of the Om(1D) gene ( L1) located in the normal recombination region of the X chromosome of D. Ananassae () were used, bringing the total number of marker loci to 10. In a previous report, a subset of these data consisting of nine loci ( L1–L9) and seven populations (BBS, BOG, CH, DAR, KATH, MAN, and PUR) was already published ().
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The Pillows (ザ・ピロウズ?, stylized as the pillows) are a Japanese alternative rock band formed in 1989. As of 2014, the group has released 19 original studio. Left to right: Yoshiaki Manabe, Sawao Yamanaka, Shinichiro Sato. Background information Origin, Japan Genres, Years active 1989–present Labels Delicious Label (2016-present), (1994-2007, 2016-present), Captain Records (1989-1991), (1991-1994), (2007-2016) Associated acts,, Kenzi & The Trips Website Members Shinichirou Sato Past members The Pillows (: ザ・ピロウズ,: Za Pirōzu, stylized as the pillows) are a Japanese band formed in 1989. The group has released 22 studio albums, several and compilations, and over 35 singles. Outside Japan, they are best known as the group responsible for the soundtrack to the series. Contents • • • • • • • • • • • • • History [ ] Formation and early years (1989–1994) [ ] In 1989, bassist for Kenzi & The Trips, left the band with former Kenzi's drummer and invited The Coin Locker Babies vocalist to form a new band. Since Yamanaka was not able to play the guitar well yet,, the guitarist of the hair metal band Persia, joined them. The Pillows were formed on September 16, 1989. The story as to where the band's name came about is that allegedly Sawao Yamanaka was hanging out at Yoshiaki Manabe's place and an English post-punk compilation record entitled hanging on Manabe's wall inspired him. Ueda and Yamanaka were the main songwriters and The Pillows released their first EPs, and in 1990 under Captain Records. In 1991 they contracted with major label and released their debut single, 'Ame ni Utaeba' in May and their first full-length record in June. In 1992 The Pillows visited England to record their second full-length album,, and filmed the promotional video for their second single, 'Kanojo wa Shisuta'. Shortly after the release of White Incarnation, Ueda left The Pillows due to disagreements with other members and musical differences. Naruto character mugen download. After a year with no activity or leadership, Sawao Yamanaka took over Ueda's leadership role and recruited Tatsuya Kashima as a replacement on bass, restarting the band. Out of respect for Ueda, Kashima was labeled as a guest musician on releases on which he performed and Ueda's bassist post has never been officially replaced. The extremely rare, released only to The Pillows fanclub, contained their first recordings with Kashima. In 1994 The Pillows changed to and the band wildly experimented between varying genres. In July The Pillows released their first album as a trio,, and one month later the single ' was released. Breakthrough and FLCL (1995–2000) [ ]. Yamanaka performing in Seattle during the Delicious Bump Tour, 2008. In March 1995 The Pillows released their fourth album,. Their subsequent single, ', was used in the romance movie. In the following year, after the release of their fifth single 'Tiny Boat', The Pillows would release a series of singles such as ', ', and 'Trip Dancer' that were featured on their breakthrough and acclaimed album. In March of the same year, due to the success of Please Mr. Lostman The Pillows released one of the album's tracks, ' as a single. Later that year, two new singles were released, ' in June and ' in November, which became one of The Pillows' most recognizable songs, being largely featured on concert set lists. In January 1998, The Pillows released their twelfth single ' and their first concert DVD '. The last three singles were featured on their sixth album, which came out in February and became one of their most well known and commercial records. Two more singles followed, 'Instant Music' and ' would both be used on their 1999 album. The Pillows Discography DownloadThe year of 1999 started with the release of Runners High and to celebrate their 10th anniversary, The Pillows released their first video clips compilation DVD, ', followed by two new singles (' and '), both featured on the second studio album release of that year,. This record pays tribute to the in songs such as 'Back Seat Dog' and 'Kim Deal' and it is the first album to feature support bassist Jun Suzuki as a replacement for Kashima. In the same year, The Pillows were approached by the studio, which licensed The Pillows' three previous albums for the soundtrack of the. The Pillows also composed two new songs for the show, 'Ride on Shooting Star' and 'I Think I Can', which were later included on their greatest hits compilation album. Due to their participation in FLCL's soundtrack, The Pillows enjoyed a popularity increase, making their western fan base grow. This allowed them to release their 'Ride on Shooting Star' single in the in 2000 and later tour that country. Early 2000s, 15th anniversary and US debut (2001–2006) [ ]. The Pillows performing at Club Quattro in 2003. After their participation on FLCL's soundtrack, The Pillows released their first greatest-hits compilation album in 2001 and also a live DVD called and a new album,. In 2002, the FLCL anime became available in the United States, giving the band more notoriety outside of their native country. In October of the same year, The Pillows' tenth studio album, was released alongside a double-CD collection of B-sides entitled, following the promotional single '. In 2003, The Pillows released their second video clips DVD, one single, 'Terminal Heaven's Rock' and one more studio album,, which was later released in the US by Geneon in 2005. To commemorate the band's 15th anniversary, The Pillows re-released their EP with additional tracks and released a string of new material, including a new EP with re-recorded tracks previously published between 1990 and 1996,, one document DVD, Walkin' on The Spiral, one new studio album,, a new single, ' and finally a tribute album,, including covers of The Pillows played by artists such as,. The Pillows in 2005. From left to right: Manabe, Sato, Yamanaka. In January 2005 The Pillows released the live DVD 916 which features footage of their 15th anniversary concert of 2004. In March 2005, The Pillows played their first show in the United States at in, followed by concerts in. In September, the band released a live DVD entitled Delicious Bump Tour in USA, featuring footage of their first tour in America with fellow band and also a new single, 'Non Fiction'. In November they released their second single of the year,'. Both singles were used on their subsequent 2006 album,. In February 2006 to help promote the My Foot tour, one track of the album, ' was released as the album's third single. The album was released in the United States in July 2006 by, with a growing international fan-base and growing domestic sales of their albums and singles over the past four years. In June, The Pillows returned to North America during their tour in support of the album My Foot, with several more dates in the U.S. And a show in. Move to Avex Trax, 20th and 25th anniversaries (2007–2014) [ ] In 2007, The Pillows released their 25th single, ' which was used in the anime series, being included on their next album,. This album marks their first release on the label. In August, the band released one more single, ', which is being used as the theme song for the Japanese version of the children's animated series. In November, the band released a five-disc singles collection,, featuring all the band's singles released under the label and 21 music videos on a DVD in the same collection. On the same day they also released their fifth live DVD, Lostman Go to America featuring footage of their 2006 American tour in support for the album My Foot. The Pillows Discography 320kbps DownloadCrack detection matlab code example. Manabe performing in Seattle during the Delicious Bump Tour, 2008. In January 2008, the band released a live DVD, Wake up! And Go!, featuring footage of their Wake Up! Tour of 2007 and a single, '. On May a new single came out, ', and along with their previous two singles, it is featured on their fifteenth studio album,. The band returned to the US starting with Los Angeles, followed by other appearances at SXSW, San Francisco, Seattle, New York City, and, where the band played for a crowd of more than 5,000 people. Still in 2008, legendary English rock band extended an offer for The Pillows to play as the opening act at their concert in Japan, but frontman Sawao Yamanaka refused their offer. In a January interview for Japanzine, Yamanaka joked that he 'turned down the offer in order to have a fun story to tell'. In 2009, in order to celebrate their upcoming 20th anniversary, The Pillows issued a series of releases called 'Late Bloomer Series'. Sasiedzi Z Piekla Rodem 1 2 DownloadAnd dont forget to subscribe and like. This really help me and motivates to work:D You can support me: EXTRA TAGGS IGNORE: funny the game game drake minecraft funny video transformers funny videos youtube mortal kombat seananners dragon ball z pokemon walkthrough spiderman star wars zelda super mario play how to play the play tyga fred ghost future gra gta my life games machinima down i want you naruto bleach amv adventure time coldplay frozen halo 2 the darkness fail comedy funny moments blood. 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Harrison CNC M400 Crusader lathe Service Manual. Approximately 41 pages dealing with the basis of the lathe, parts assembly drawings, end float and gib adjustment, replacement of worn bearings, worn ball screws, belts and pulleys, micro switches and encoder settings and replacement. Download >> Read Online >>..... Old lathe manuals harrison m400 lathe manual pdf harrison l6 metric harrison lathe manual pdf rutland latheharrison m390 lathe manual harrison m540 lathe harrison lathe serial numbers MODEL L5A (11″). MODEL L5 (9″). PDF COPY of the Harrison Lathe Operation, Installation & Maintenance Manual, including pictorial parts lists. MODEL 140 A manual is available for this Harrison lathe Named after its swing of 140 mm (11 inches) the Harrison 140 was produced late in the life of the L5A/11-inch 28 Jul 2018 Harrison 140 Lathe. User manual and service reports available. The lathe weighs over 528kg and the buyer would need to arrange for aHarrison lathes – 1940s to the late 1960s including versions badged as “DoAll” Manuals are available for most Harrison lathes Model 140 L5 Accessories Rotary Machine Tools: Harrison 155 Centre Lathe For Sale. Try here Items 1 – 10 of 23 Machinery Manuals| Parts Lists| Maintenance Manual| Service Instructions| Schematics: Harrison – Lathe Manuals Milling Machines Shears 19 Nov 2015 21 Mar 2015 Doubleboost do you have a reducing bush with your new 140 you. To a “Harrison L6 Manual” you posted a PDF copy of a manual cover, •. Spares repair parts and operation and maintenance manuals are available for most colchester clausing lathes colchester lathe serial numbers colchester home bantams original bantams 800 1600 2000 modern chipmaster student 1800 mascot 1600 student master mk 1 mk 2 student 3100 master original 1930s 40s master 2500 triumph mk 1 mk 2 triumph 2000 mascot 1950s mastiff 1400. Manuals available for harrison m300 harrison m400 harrison m500 lathes and also colchester triumph colchester mastiff colchester mascot lathes. Starter 4 1 siemens software. To create reliable machines, our new SIRIUS 3RW5 soft starters offer you intelligent functions such as automatic parameterization, pump cleaning, and condition monitoring. Highlights In which soft starter technology do I need to invest in the next 6 months? Harrison M400 Lathe Manual PdfHarrison M400 Lathe Owners ManualMachine tool manuals operators service maintenance lubrication electrical hydraulic manualsbudget machinery used machine tool sales import export england. Nearest town or city: Saratoga Directions: From Los Gatos - go west on highway 9 until the junction with Skyline Boulevard. Check out the guide books and go climb! Go left on Skyline for about 5-10 minutes. |
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